

Our methods
In our lab, we use a broad spectrum of molecular biological and biochemical methods.

Live/ Dead staining
Analysis of cell viability
Assessment of cytotoxic effects

Flowcytometry
Detection of cell surface markers
Characterization of cells

Zymography
Quantification of MMPs
Determination of proteolytic activity

Immunohistochemistry
Detection of target proteins
Characterization of cells/ tissue

qRT-PCR/ siRNA
Analysis of gene expression pattern
Investigation of protein function

(cell-based) ELISA
Detection of cell surface markers
Quantification of protein release

Immunofluorescence
Detection of target proteins
Assessment of cell morphology

Cell migration assays
Testing of chemoattractive drugs
Analysis of in vitro wound healing

Differentiation assays
Investigation of stem cell properties
Evaluation of therapeutics

Histo(morpho)logy
Assessment of matrix composition
Investigation of cellular properties